The expression of DcR3 in gastrointestinal tumors / 肿瘤

ABSTRACT

Objective: To study the expression of decoy receptor 3 (DcR3) in gastrointestinal tumors and elucidate the relationship between DcR3 and gastrointestinal tumors. Methods: The human colon carcinoma cells (SW480), gastric cancer cells (SGC7901), hepatoma carcinoma cell (HepG2) and human fibroblasts (3T3) were cultured in vitro. The expression level of DcR3 mRNA was determined by RT-PCR. The level of DcR3 protein expression was detected by Western blotting. Results: The mRNA and protein expression levels of DcR3 were higher in the SW480 cells than those in SGC7901, HepG2, and 3T3 cells. The difference was significant (P <0.05). Conclusion: Over-expression of DcR3 gene in the colon carcinoma cells may be related with the initiation and development of colon cancer.