Expression of PNMA5 in hepatocellular carcinoma tissues and its function / 肿瘤

ABSTRACT

Objective: To study the expression of function-unknown PNMA5 gene in hepatocellular carcinoma (HCC) and its relationship with proliferation of HCC. Methods: PNMA5 was over-expressed in HCC analyzed by gene expression profiling. We investigated the expression of PNMA5 through RT-PCR and real-time fluorescent quantitative PCR (RFQ-PCR) in normal liver tissues, HCC, and adjacent non-cancerous liver tissues (non-HCC). PNMA5 gene was silenced in Focus and PLC liver cancer cells by using RNA interference technology. The changes in cell growth curve were quantitatively analyzed by performing CCK-8 experiment after silencing the endogenous PNMA5 gene expression. Results: PNMA5 was mainly expressed in testis, ovary, and brain tissues out of the 14 kinds of normal human tissues. PNMA5 mRNA was significantly up-regulated in 42% (5/12) HCC specimens, which was 2-fold higher than that in non-HCCs (P < 0.05). Interestingly, the growth of Focus and PLC cells was markedly inhibited after silencing PNMA5 gene expression (P < 0.01), and the number of tumor cells progressing through G1 and entering S phase was significantly decreased as compared with the control cells transfected with siRNA-NC (P < 0.01). Conclusion: PNMA5 plays an important role in maintaining the malignant phenotype of HCC. It may be a novel tumor-testis associated gene. Further investigation of its function may help to find the new mechanism for the HCC tumorigenesis. PNMA5 may become a new candidate target in HCC treatment.