The expression of copper-transporting P-type adenosine triphosphatase in ovarian cancer and its correlation with chemotherapy resistance / 肿瘤

ABSTRACT

Objective: To investigate the expression of copper-transporting P-type adenosine triphosphatase (ATP 7B) in the ovarian cancer and evaluate its relationship with chemo-resistance and the clinicopathologic parameters of ovarian carcinoma. Methods: Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of ATP 7B in 36 cases of ovarian carcinoma, 10 cases of benign ovarian tumor, and 10 cases of normal ovarian tissue. Results: The positive expression rate of ATP 7B in ovarian carcinoma was 38.9% significantly higher than that in benign ovarian tumor (0%) and normal ovarian tissue (0%) (P < 0.01). There was a significant correlation between ATP 7B expression and differentiation degree. ATP 7B expression was significantly higher in poorly differentiated carcinoma than that in well and moderate differentiated carcinoma (P < 0.05). The expression of ATP 7B was more positive in patients who received chemotherapy before surgery than those who did not receive chemotherapy. The difference was significant (P < 0.05). The response rate of ATP 7B-positive patients to chemotherapy was significantly lower than the ATP 7B-negative patients (28.6% vs 72.7%, P < 0.05). Conclusion: These data suggested that ATP 7B plays an important role in the multidrug resistance of the ovarian carcinoma to Pt-based chemotherapy.