Screening for an animal model of human lung cancer with highly metastatic potential as well as establishment its corresponding cell line / 肿瘤

ABSTRACT

Objective: To establish an animal model of human lung cancer in NOD/SCID mice and its cell line with highly metastatic potential and observe their correlated biological features in metastasis so as to provide an important useful tool for studying the metastatic mechanism, diagnosis, prevention and therapy of lung cancer. Methods: Human lung cancer cells SPC-A-1 were inoculated subcutaneously into NOD/SCID mice, metastatic tumor masses in lung were harvested from curative resection of subcutaneous tumor, and re-implanted into NOD/SCID mice for the second round of in vivo selection. Besides resection of subcutaneous tumor, the same manipulation was triplicately repeated. Eventually, we established a highly metastatic animal model of human lung cancer in NOD/SCID mice and its cell line with highly metastatic behavior. The cell growth behavior, metastasis, morphological characteristics of the implanted tumors were observed by light microscopy. The biological features of the highly metastatic cell line were investigated. Results: The lung metastasis rate of SPC-A-1 cells was 66. 7% after curative resection of subcutaneous tumor in the first round in vivo screening. An animal model of human lung cancer with 100% lung metastasis and its cell line with unique metastatic characteristics were established by in vivo 4-round repeated screening. The cell growth behavior and karyotype analysis showed that SPC-A-1 cell line maintained the biological characteristics of primary human lung adenocarcinoma. Conclusion: A highly metastatic animal model of subcutaneous implanted human lung cancer and its cell line are successfully established by in vivo screening. Our study provides an ideal animal model for research of prevention of lung cancer and experimental therapy against metastasis.