Relationship between the adnormal expression of KAI 1 gene and carcinogenesis and development of thyroid papillary carcinoma / 肿瘤

ABSTRACT

Objective: To investigate the relationship between the expression of KAI 1 gene and the carcinogenesis and development of thyroid papillary carcinoma (PTC). Methods: The expressions of KAI1 mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical method (Envision™) in 59 cases of malignant and benign thyroid tissues, respectively. Their correlation with clinical pathological parameters were analyzed. Results: The level of KAI1 mRNA expression was 3.59 ± 1.57 in PTC, and the expression of KAI1 protein was positive in 24 cases (66.67%). Both the mRNA and the protein expressions of KAI1 were significantly higher than those of follicular adenoma (P <0.01, P <0.05), multinodular goiters (P < 0.01, P < 0.01) and normal thyroid tissues (P < 0.01, P < 0.01). The expressions of KAI1 mRNA and protein had significant correlation with the lymph node metastasis and the tumor size of patients with thyroid papillary carcinoma (P < 0.05). The correlation between KAI1 mRNA and protein are significant positive (r = 0.486, P < 0.01). Conclusion: The abnormal expression of KAI1 mRNA and protein are related with the carcinogenesis and development of thyroid papillary carcinoma. It provides the basis for evaluating the lymph node metastasis and prognosis of thyroid papillary carcinoma.