The interaction between HSP 90 and survivin in human breast cancer cells and its underlying mechanism / 肿瘤

ABSTRACT

Objective: To study the interaction between heat shock protein 90 (HSP90) and the survivin in human breast cancer cells and its effect on proliferation and apoptosis of tumor cells. Methods: The human breast cancer MDA-MB-231 cells were treated with an HSP90 inhibitor, geldanamycin (GA). The expression of phosphorylated signal transducers and activators of transcription 3 (P-STAT3) and survivin protein were detected by Western blotting. The mRNA transcription of sruvivin was determined by RT-PCR. The cell proliferation was measured by MTT assay. Apoptosis was examined by flow cytometry. Results: HSP 90 inhibitor GA reduced the expression of P-STAT3 and survivin, inhibited the proliferation, and induced apoptosis of human breast cancer MDA-MB-231 cells. Conclusion: HSP90 affected the transcription of survivin mRNA through activation of the JAK-STAT3 signaling pathway. Surviving-HSP90 interaction contributed to the promotion of the high-proliferation and low-apoptosis malignant behaviors of human breast cancer MDA-MB-231 cells.