Relation between ionization and the expressions of HIF-1α and VEGF in anoxia condition / 肿瘤
Tumor
;
(12): 545-548, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-849545
ABSTRACT
Objective:
To investigate the effect of ionization on the expression of HIF-1 α and VEGF in anoxia condition, in order to search an effective method for improving the radiosensitivity.Methods:
HepG2 hepatoma carcinoma cells were divided into four groups the control group; the hypoxia group; the radiation group; the hypoxia and radiation group. The cell apoptosis was detected by fluorescence microscope. The cell viability was analyzed by MTY method. And we measured the expressions of HIF-1 α mRNA and VEGF mRNA by RT-PCR.Results:
Cell apoptosis no apoptosis was observed in the control group; few apoptosis occurred in the hypoxia group; bulk apoptosis was observed in the radiation group; less apoptosis occurred in the hypoxia and radiation group than that in the hypoxia group. The order of surviving fraction was the control group > the hypoxic group > the hypoxia plus radiation group > the radiation group; The order of the expression of HIF-1 α mRNA the hypoxia plus radiation group > the hypoxic group > the radiation group, no significant difference was observed between the radiation group and the control group; The order of the expression of VEGF mRNA the hypoxia plus radiation group > the hypoxic group > the radiation group > the control group.Conclusion:
The expression of HIF-1α induced by hypoxia can prevent hepatoma cells from the damage of radiation. The HIF-1 α decreases the radiosensitivity through inducing VEGF expression in the HepG2 cell line.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Tumor
Año:
2007
Tipo del documento:
Artículo
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