HPV-16 E6 siRNA associated with hIL-24 gene induces apoptosis of human cervical cancer Ca Ski cells / 肿瘤

ABSTRACT

Objective: To evaluate the apoptosis-inducing effect of HPV-16 E6 siRNA associated with hIL-24 gene on human cervical cancer Ca Ski cells. Methods: HPV-16 E6 siRNA and hIL-24 gene eukaryotic expression vector alone or in combination were transfected into human cervical cancer Ca Ski cells. The expression level of HPV-16 E6 mRNA was detected by RT-PCR. The expression of tumor suppressor protein p53 was analyzed by western blot. The apoptosis of Ca Ski cells was analyzed by flow cytometry analysis. Results: The expression level of HPV-16 E6 mRNA decreased in Ca Ski cells in all the three groups after transfection with HPV-16 E6 siRNA and hIL-24 gene eukaryotic expression vector. The decrease was significant in co-transfection group ( P < 0. 05 ). The expression of tumor suppressor protein p53 and the apoptosis rate of Ca Ski cells increased in the three groups and the increase was significant in co-transfection group ( P < 0.05 ). Conclusion: Co-transfection of Ca Ski cells with HPV - 16 E6 siRNA and hIL-24 gene inhibits the expression of tumor gene HPV E6, recovers the activity of tumor suppressor protein p53, and induces the apoptosis of Ca Ski cells. HPV - 16 E6 siRNA associated with hIL-24 gene significantly increases the apoptotic rate of Ca Ski cells. They have synergistic effects.