Comparison of two preparation methods of liver whole-organ decellularized matrix / 解放军医学杂志

ABSTRACT

Objective To compare the two different decellularization methods for preparation of liver whole-organ decellularized matrix for providing an experimental basis for screening more effective method. Methods Liver whole-organ decellularized matrix was prepared by two different decellularization methods using sodium dodecylsulfate (SDS) and Triton X-100+SDS (Tx+SDS) as main detergent respectively. The three-dimensional shape, vascular structure, histological results and immunofluorescence images were observed, and the contents of residual DNA, collagen and growth factors (HGF, bFGF) were determined by ELISA. Results Liver whole-organ decellularized matrix was successfully prepared by both methods, as the cellular and nuclear components were removed successfully and the extracellular matrix (ECM) protein was preserved. The Tx+SDS method preserved the vascular structure of the liver, and the SDS method resulted in damage to the vascular structure. Compared with SDS method, the Tx+SDS method preserved more ECM protein, including almost 100% of collagen, and 58.5% of HGF and 42.5% of bFGF. Conclusion The liver whole-organ decellularized matrix could be well prepared by Tx+SDS method, and it may provide an effective scaffold for the study of tissue engineered liver and regenerative medicine.