Chemical comparison of Salvia miltiorrhiza phloem and xylem based on fingerprint analysis and quantitative analysis of multi-components / 中草药

ABSTRACT

Objective: To comprehensively compare and evaluate the composition of Salvia miltiorrhiza phloem and xylem samples based on fingerprint analysis and quantitative analysis of multi-components. Methods: In the present study, an accurate and reliable fingerprint approach was developed using high performance liquid chromatography for chemical comparison ofphloem and xylem samples of S. miltiorrhiza. Furthermore, eight bioactive compounds including four salvianolic acids and four tanshinones in S. miltiorrhiza phloem and xylem samples were simultaneously quantified. Moreover, chemometrics methods were performed to compare and discriminate the phloem and xylem based on the quantitative data. Results: The specific fingerprints of phloem and xylem of S. miltiorrhiza were obtained, and a total of 10 common peaks were marked. The quantitative and chemometrics analysis results indicated the content of chemical components in phloem and xylem samples of S. miltiorrhiza were notably different. Obviously, the content of tanshinones were notably different between phloem and xylem samples. The content of tanshinones were significant higher in phloem compared with xylem in S. miltiorrhiza. Conclusion: The fingerprint analysis and quantitative analysis of multi-components could be a well-acceptable strategy for chemical comparison of S. miltiorrhiza phloem and xylem.