Simultaneous determination of six components from Rumex chalepensis with different growth years and harvesting periods by HPLC / 中草药

ABSTRACT

Objective: To establish a method for the determination of six components from Rumex chalepensis Mill.. Methods: The contents of chrysophanol-8-O-β-D-glucoside, emodin-8-β-D-glucoside, nepodin, emodin, chrysophanol, and physcion were simultaneously determined by HPLC. The mobile phase was methanol-0.1% formic acid, gradient elution, flow rate of 1 mL/min, column temperature of 25 ℃, injection volume of 5 μL, detected by Agilent Extend-C18 (250 mm × 4.6 mm, 5 μm) and diode array detector at 254 nm wavelength. Results: The content of chrysophanol-8-O-β-D-glucoside, emodin-8-β-D-glucoside, nepodin, emodin, chrysophanol, and physcionhad good linear relationship in the ranges of 208—3 120, 22.40—336.35, 178.9—2 908.8, 16.7—250.8, 104.4—1 566.0, 45.2—677.7 ng, respectively. The average recovery rates were 97.66%, 97.10%, 98.78%, 97.38%, 102.48%, and 95.51% (n = 6). The contents of chrysophanol-8-O-β-D-glucoside, emodin-8-β-D-glucoside, nepodin, emodin, chrysophanol, and physcion in 16 batches of R. chalepensis. were determined in the range of 0.6—7.1, 0—2.7, 1.0—6.5, 0.1—0.6, 0.7—4.3, and 0.1—0.4 mg/g, respectively. Sample contents of different growing years, harvesting dates, and plots were compared and analyzed. Two-year-old R. chalepensis. was collected in early spring or late summer and early autumn. The total content of six components was 12.2 mg/g, which was relatively high. Conclusion: The established method can be used for simultaneous determination of six components from R. chalepensis, and determine the harvesting time and season of R. chalepensis, which provides a scientific basis for the formulation of quality evaluation criteria of R. chalepensis.