Spectral-effect relationship between HPLC fingerprint and cell proliferation and differentiation of crude and sweated Dipsaci Radix / 中草药

ABSTRACT

Objective: To study the spectrum-effect relationships of fingerprints of sweated and crude Dipsaci Radix on cell proliferation and differentiation, and find out the material basis of efficacy before and after sweating in order to provide the basis for the impact of the efficacy. Methods: The DAD detector was used to establish HPLC fingerprints of sweated and crude Dipsaci Radix, and the relationship between the spectrum and efficiency was established by gray relational analysis. Results: The chemical composition of peaks 14 and 4 were highly correlated with the proliferation and differentiation of osteoblasts and the proliferation of MG-63 cells. The correlations were all above 0.7. The three pharmacophore indicators associated much with the characteristic peaks 14, 4, 6, 16, 13, 11, and 5. Combined with the previous analysis, these peaks represented respectively asperosaponin VI, chlorogenic acid, loganin, asperosaponin IV isomers, dipsacoside X, chlorogenic acid C, and caffeic acid. Conclusion: Asperosaponin VI, chlorogenic acid, loganin, asperosaponin IV isomers, dipsacoside X, chlorogenic acid C, and caffeic acid may be the main material basis for the effect of cell proliferation and differentiation, thus affecting its efficacy.