Effect of Isaria felina on immune function of immunosuppressed mice / 中草药

ABSTRACT

Objective: To investigate the effects of Isaria felina (IF) on immune function in mice with immunosuppression induced by cyclophosphamide. Methods: The kunming mice were randomly divided into control group, model group, positive group (lentinan, 200 mg/kg), high, medium, and low dose groups of IF (400, 200, 100 mg/kg). Except for the control group, the other five groups were intraperitoneally injected with cyclophosphamide to establish an immunosuppression mouse model. The drug was administered once a day for a total of 10 d. Then the body mass growth, liver index, thymus index, spleen index and blood routine indexes were examined; The carbon profile method and the delayed immune response (DTH) was used to determine the non-specific immune function and the cellular immune function in mice; The content of TNF-α in serum of mice was determined by double antibody sandwich ELISA. Results: Compared with the control group, the growth value of body mass, spleen index, white blood cells, red blood cells and other blood routine indexes, phagocytic index, plantar thickness difference and TNF-α content of model group were significantly reduced (P < 0.05), which suggested that the establishmeng of immunocompromised mouse models induced by cyclophosphamide was successful. Compared with the model group, spleen index, white blood cells, red blood cells and other blood routine indexes, phagocytic index, plantar thickness difference and TNF-α content in each dose group of IF were significantly increased (P < 0.05). Conclusion: I. felina has immunoprotective effects on mice with immunodeficiency induced by cyclophosphamide.