Rapid identification on chemical compounds in roots of rheum nobile based on uplc-esi-hrmsn / 中草药

ABSTRACT

Objective A rapid and efficient UPLC-ESI-HRMSn method was developed and applied to rapidly identify the components in Rheum nobile. Methods With 100% methanol as extract preparation for solution,the extract was separated on an ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm), and eluted with a gradient of methanol-water that containing 0.1% formic acid. Chemical constituents were separated and investigated by UPLC-ESI-HRMSn in both positive and negative ion modes. Combined with accurate mass characteristic fragments, retention time and previous literature database, the structures of compounds were identified or tentatively characterized. Results A total of 34 compounds including four anthraquinones, 15 phenolic acid derivatives, 11 tannin precursor and tannins and four organic acid were identified. Twenty-seven compounds were reported for the first time from R. nobile. Conclusion The analytical method of UPLC-ESI-HRMSn was valuable for rapid identification of unknown constituents of R. nobile, which supplies a clear material basis for further study.