Preparation of hydrophobic chitosan from Catharsius molossus and its hemostatic effect / 中草药

ABSTRACT

Objective To prepare hydrophobic chitosan from Catharsius molossus and study its hemostatic effect. Methods The chitosan from C. molossus was used as material to carry out hydrophobic modification and characterization by acylation with lauric anhydride. At the same time, the hemostatic effect was studied by in vitro agglutination test and mouse liver wound experiment. Results The prepared hydrophobic chitosan had a hydrophobic group substitution degree of (11.69 ± 0.14)%, which was higher than that of commercially available hydrophobic chitosan. The in vitro agglutination experiment showed that it could coagulate in (3.42 ± 0.39) s when mixed with blood at a concentration of 6.0 mg/g, which was better than chitosan and hydrophobically modified commercial chitosan. The mouse liver wound experiment showed that chitosan from C. molossus could shorten hemostatic time significantly compared with chitosan and hydrophobically modified commercial chitosan. Conclusion Hydrophobically modified chitosan can significantly improve the coagulation performance of chitosan, and the hydrophobic chitosan from C. molossus is excellent, and it is expected to be a new hemostatic material with great potential.