Resveratrol inhibits ovarian cancer cell growth and Wnt signaling pathway by regulating SIRT1 / 中草药

ABSTRACT

Objective: To study the effects of resveratrol on cell proliferation and apoptosis of A2780 cells, and explore its mechanism. Methods: MTT assay was used to detect inhibition rate of A2780 cells with resveratrol (50, 100, 200, and 400 μmol/L); Resveratrol + pcDNA3.1 group (transfected pcDNA3.1), and resveratrol + pcDNA3.1-SIRT1 group (transfected pcDNA3.1-SIRT1) were transfected into A2780 cells by liposome method, and treated with resveratrol (200 μmol/L); The mRNA levels of SIRT1, β-catenin, and c-Myc were detected by qRT-PCR. The protein expression of SIRT1, β-catenin, and c-Myc were detected by Western blotting. The apoptosis rate of each group was detected by flow cytometry. Results: Compared with the blank control group, the inhibition rate and apoptosis rate were significantly increased (P < 0.05) in A2780 cells with resveratrol (50, 100, 200, and 400 μmol/L), and the mRNA and protein levels of SIRT1 were significantly decreased (P < 0.05) in the resveratrol (200 μmol/L) group, which could inactivate the Wnt signaling pathway. Overexpression SIRT1 reversed the inhibitory effect of resveratrol on A2780 cell proliferation and the inactivation of Wnt signaling pathway. Conclusion: Resveratrol can inhibit ovarian cancer cell proliferation and promote apoptosis in ovarian cancer cells by regulating SIRT1, and its pro-apoptotic mechanism may be relate to inactivated Wnt signaling pathway, which will provide a basis for resveratrol treatment of ovarian cancer.