Hydrolysis mechanism of aconitine and deoxyaconitine in Aconitum soongaricum / 中草药

ABSTRACT

Objective To determinate the hydrolysis products of two ingredients in Aconitum soongaricum under different heating conditions by liquid chromatography-mass spectrometry method, analyze its hydrolysis change rule, and verify it in A. soongaricum, analyze the changes of alkaloid before and after processing, and summarize the change rules and processing hydrolysis mechanism, so as to provide theoretical basis for the processing parameters of A. soongaricum. Methods Taking two kinds of main chemical constituents in A. soongaricum, aconitine and deoxyaconitine as the research object, the change rule of alkaloid monomer variation under different heating conditions was summarized combined with HPLC and HPLC-MS results, which was verified in different processed products in A. soongaricum. Results There was the significant change in the content of aconitine and deoxyaconitine after different heating time, the content of the mainly products from aconitine hydrolysis was increased with the increase of heating time, and aconitine and deoxyaconitine were gradually disappeared, eventually not detected. Conclusion The processing parameters of A. soongaricum were preliminarily determined as heating for 5-6 h, and the hydrolysis of aconitine and deoxyaconitine producing new hydrolysis products, thus achieving the effect of reducing toxicity and increasing efficiency.