Quality research of Gastrodiae Rhizoma with UPLC fingerprint and content determination of multicomponent / 中草药

ABSTRACT

Objective To establish the analysis method of UPLC fingerprint and simultaneous determination of five active components (gastrodin, 4-hydroxybenzyl alcohol, parishin B, parishin C, and parishin A). Methods The analysis was performed on a Waters Acquity with a Waters ACQUITY UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) column. The gradient mobile phase was acetonitrile and 0.1% formic acid-water solution, flow rate was set at 0.3 mL/min, the injection volume was 0.2 μL and the temperature of column was 35 ℃. The detection wavelengths were set at 270 nm (fingerprint), 220 nm (content determination). The 28 batches of samples fingerprint were analyzed by similarity evaluation, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA). The identification and content determination of the main common peaks were carried out by comparison with reference. Results We set up the common mode of the fingerprint with 20 common peaks, and six of them were identified, which were gastrodin (1), 4-hydroxybenzyl alcohol (3), p-hydroxybenzaldehyde (7), parishin B (14), parishin C (15), and parishin A (18). The similarities among 28 samples were all above 0.90. PCA was used to divide the samples into five groups, and six different substances were found. The content of the same component and the fluctuation range among different components were all different. The content of the five components of each origin was Guizhou 2.52% > Yunnan 2.49% > Shaanxi 2.33% > Hubei 2.10% > Zhejiang 1.90% > Anhui 1.65%.Conclusion The establishment of UPLC fingerprint and simultaneous determination of five active components provide a reference for quality control of Gastrodia Rhizoma.