Establishment of quality evaluation model of material foundation-efficacy- bioactivity of Pinelliae Rhizoma / 中草药

ABSTRACT

Objective To establish the quality evaluation model of material foundation-efficacy-bioactivity of Pinelliae Rhizoma by investigating the relationships between the total organic acids of Pinelliae Rhizoma and the antitussive effect, the HPLC fingerprint of total organic acid and the antitussive effect, the total organic acids and the antitussive potency, respectively. Methods The content of total organic acids in Pinelliae Rhizoma was determined by potentiometric titration. The total organic acid fingerprints were established by HPLC. The cough relieving effect was studied by the concentrated ammonia water cough model, and the spectral-effect relationship between total organic acids and antitussive effect was established by the grey correlation analysis. Based on the mice concentrated ammonia water cough model, the determination method of the cough potency of Pinelliae Rhizoma was established. The correlation among the total organic acids, the antitussive effect and the antitussive potency was analyzed by SPSS 18.0 Software. Results The total organic acids of Pinelliae Rhizoma had a significant inhibitory effect on mice cough induced by concentrated ammonia. The antitussive effect was enhanced with the increase of the content of total organic acids. In the HPLC chromatogram, the chemical components represented by chromatographic peaks of 4, 6 (succinic acid), and 12 showed a strong correlation with the antitussive effect. The cough efficacy mean value of ten batches of Pinelliae Rhizoma was (753.72 ± 58.18) U/g. Combined with the quantitative analysis of total organic acids, there was a significant positive correlation between them, showing an increase in the antitussive efficacy with an increase of the content of total organic acids. Conclusion This study not only revealed the material foundation of the anti-tussive effect of Pinelliae Rhizoma, but also clarified the correlation among total organic acids, antitussive effect and bioactivity. Finally, it can improve the quality standards of Pinelliae Rhizoma and provide a scientific basis for the construction of a new model for quality control of Pinelliae Rhizoma.