Quality evaluation of Flos Sophorae Immaturus from different habitats by HPLC coupled with chemometrics and anti-oxidant ability / 中草药

ABSTRACT

Taking Flos Sophorae Immaturus (FSI) samples from different habitats in two years as materials, to establish the HPLC fingerprint of FSI, determine the contents of five kinds of flavonoids by HPLC, and evaluate the quality of FSI from different habitats according to the total flavonoids contents and anti-oxidant ability. Methods Total flavonoids, rutin, quercetin, genistein, kaempferol, isorhamnetin, DPPH, hydroxyl radicals, superoxide anion radicals, lipid peroxidation of 54 pieces of FSI samples were determined by HPLC and spectrophotometry. The quality of FSI from different areas were analyzed by similarity, clustering analysis, and principal component analysis method. Results HPLC fingerprint was established and all samples could be separated well. All the indexes of the methodological investigation arrived the requirements, and 28 common peaks in fingerprints were found, five characteristic peaks whose total peak area is more than 80% of the common peak area were identified, respectively. Based on the contents of total flavonoids, five flavonoids, and four anti-oxidative ability, 54 pieces of samples were analyzed by cluster analysis and principal component analysis. The results were the same, which did not show obvious geographical characters from different samples. Conclusion HPLC combined with chemometric method and anti-oxidant ability is accurate and comprehensive for the quantitative analysis of FSI. The quality of FSI samples from the same growing areas in different growing years and from different growing areas in the same growing year were different, which showed that the quality of FSI was affected by climate. Different samples from the same growing area in the same year were different, which showed that the quality of FSI also was affected by key producing technology.