Genetic diversity analysis of Pholidota cantonensis in Zhejiang based on SRAP molecular marker technique / 中草药

ABSTRACT

Objective: To study the genetic diversity of medicinal plant Pholidota cantonensis Rolfe. By SRAP molecular marker technique, and to lay foundations for the protection of this species. Methods: Seven pairs of primers were screened from 81 SRAP primer pairs, and used for SRAP-PCR; The genetic diversity of 68 P. cantonensis materials from five natural populations in Zhejiang Province was applied to analyze. Results: Totally 197 reproducible bands were obtained, and 190 bands of which were polymorphic bands. Each primer pairs contributed 27.29 band on average, and the effective number of allele (Ne) was 1.238 1. At the species level, the percentage of polymorphic bands (PPB) was 96.45%, and the Nei’s genetic diversity (H) and the Shannon’s information index (I) were 0.188 2 and 0.312 5, respectively. At the population level, the PPB ranged from 30.46% to 46.19% with an average PPB of 40%, their H and I were 0.089 1-0.155 9 and 0.138 7 - 0.234 9, with average levels from 0.120 8 to 0.186 4, respectively. The coefficient of gene differentiation (Gst) for five populations was 35.81%, and the gene flow (Nm) was 0.896 3. The Unweighted Pair Group Method with Arithmetic (UPGMA) mean clustering results indicated that 68 samples were divided into five groups, and P. cantonensis plants from the same population were clustered into the same clade. Conclusion: P. cantonensis showed a high level of genetic diversity, and its genetic differentiation as well as gene flow were found among the five populations; However, but the genetic differentiation happened mainly within each population.