Effects of Huanbei Zhike Recipe on cough variant asthma model guinea pig based on 1H-NMR metabolomics / 中草药

ABSTRACT

Objective To investigate the mechanism of action of Huanbei Zhike Recipe on the cough variant asthma model (CVA) of guinea pigs by 1H-NMR metabolomics methods. Methods The CVA model was induced by ip 4% OVA and ip 2% Al(OH)3 in guinea pigs abdominal cavity, and the effects of Huanbei Zhike Recipe (5, 10, and 20 g/kg) on the cough induced by capsaicine was investigated. After the administration, the lung of right middle lobe in guinea pigs were taken out and determinated by 1H-NMR, and multivariate statistical analysis was used to investigate the intervention effects of Huanbei Zhike Recipe on CVA. Results The cough latency of guinea pigs can be significantly prolonged and the frequency of cough were reduced (P < 0.01), and the inflammatory factors in lung lavage fluid were obviously reduced (P < 0.05). Compared with the blank group, the levels of leucine, isoleucine, valine, alanine, α-glucose, acetic acid, glutamic acid, methionine, pyruvic acid, succinic acid, 3-hydroxybutyrate (3-HB), GSSG, creatine, ethanolamine, cholic acid, glycine, glycerophosphoryl choline (GPC), glutamine, betaine, phosphorylethanolamine (PE), β-glucose, and arginine were increased, while the levels of lactate and methyl-phosphate were decreased. These 24 potential metabolites could be reversely regulated in different degree after treatment with Huanbei Zhike Recipe. Conclusion Huanbei Zhike Recipe could prevent CVA diseases likely through the regulation of energy metabolism and amino acid metabolism.