Establishment of fingerprint of Astragali Radix polysaccharides based on endo-1,3-β-glucanasehydrolysis and identification of Astragali Radix of different germplasm resources / 中草药

ABSTRACT

Objective Enzymatic hydrolysis of Astragali Radix polysaccharides from different germplasm resources Astragalusmembranaceus var. mongholicus (MG) (cultured and natural) or Astragalusmembranaceus (MJ) (cultured and natural) was carried out by the best enzymolysis conditions of endo-1,3-β-glucanase. Saccharide fingerprints were obtained for the identification and evaluation of the germplasm resources of Astragali Radix by Fluorophore-assisted Carbohydrate Electrophoresis (FACE). Methods The data were analyzed by principal component analysis and t test using SMICA software to distinguishdifferential sugar segments among different germplasm resources of Astragali Radix. Results Pentasaccharide and hexasaccharide of endo-1,3-β-glucanasehydrolyzate could be used as differentiated saccharide fragments between natural MG and MJ.Trisaccharide, tetrasaccharide, and pentasaccharide could be used as differentiated saccharide fragments to distinguish the cultured MG and MJ.The pentasaccharide and hexasaccharide can be used as differential fragments to distinguish MJ (culturedandnatural). Conclusion Thepolysaccharide products degraded by endo-1,3-β-glucanase can well distinguish Astragali Radix species (MG and growth mode (cultured and natural Astragali Radix). This study laid the foundation for the quality evaluation of Astragali Radix and screening of active oligosaccharides.