Multi-components determination and fringerprints analysis by HPLC in Chaenomeles thibetica / 中草药

ABSTRACT

Objective To establish a HSDE-HPLC-DAD-ESI-TOF/MS method for the rapid identification of chemical ingredients for Chaenomeles thibetica, and establish the multi-component quantitative fingerprint to evaluate its quality. Methods The separation was developed via an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm, 5.0 μm) with a gradient elution at a temperature of 25 ℃, the acetonitrile was used as the mobile phase A and 0.5% glacial acetic acid and 10 mM ammonium acetate solution was used as the mobile phase B with a flow rate of 0.8 mL/min, and the detection wavelength was 280 nm. ESI-TOF/MS was used for the identification of chemical ingredients in C. thibetica. The fingerprint of C. thibetica was established based on the quantitative detection of multi-components. Results The HPLC fingerprint of C. thibetica has 30 common peaks, nine ingredients (chlorogenic acid, vanillic acid, caffeic acid, veratric acid, rutin, quercitrin, hyperin, oleanolic acid, and uosolic acid) of C. thibetica were identified, and the contents of them were determined. Similarity analysis shows that the similarity of 10 batches of C. thibetica is relatively close, while the similarity between C. thibetica and other varieties' is quite different. Conclusion HSDE-HPLC-DAD-ESI-TOF/MS method can be used for the rapid extraction and analysis of C. thibetica fresh samples. On this basis, the fingerprint analysis combined with similarity analysis can be used for quality evaluation of C. thibetica.