Exploration of Ento-II plastic on analgesic and promoting blood circulation and removing stasis / 中草药

ABSTRACT

Objective To study the analgesic, activating blood and resolving stasis effect of Ento-II plastic. Methods Hot plate procedure and torsion body method were used as analgesic experimental model to observe the analgesic effect of Ento-II plastic. The acute blood stasis rats experimental model was established through rat subcutaneous injection of high-dose adrenaline hydrochloride and soaked in ice water. Then, the blood stasis focal cerebral ischemia-reperfusion models were established by suture method on the rats. The hemorrheologic indicators were determined. Results Compared with control group, Ento-II plastic could significantly prolong pain thresholds in mice at 30, 60, and 90 min after final administration (P < 0.01). Ento-II plastics 20, 10, and 5 mg/kg dose groups could reduce aceticacid-induced mice writhing times and prolong the incubation period (P < 0.01). The experimental result of rats blood stasis focal cerebral ischemia-reperfusion at 24 h after reperfusion showed that Ento-II plastics 6.67, 3.33, and 1.67 mg/kg dose groups could obviously reduce the neurological function score and whole blood viscosity (P < 0.01). 3.33 mg/kg dose group could significantly reduce plasma viscosity, erythrocyte aggregation index, and casson viscosity (P < 0.05). The effect were similar to that of ligustrazine. 6.67 and 3.33 mg/kg dose groups could significantly reduce casson viscosity (P < 0.01). Conclusion Ento-II plastic has obvious analgesic effect and activating blood and resolving stasis effect.