Terpenes from Carpesium abrotanoides and their cytotoxic activities / 中草药

ABSTRACT

Objective To study the terpenes from Carpesium abrotanoides and their cytotoxic activities. Methods The separations and purifications were taken by column chromatography over normal phase silica gel, preparative thin-layer chromatography and preparative HPLC. Their chemical structures were elucidated through spectroscopic analyses (NMR). The in vitro cytotoxic activities of the isolated compounds were studied by MTT method. Results Six eudesmane-type sesquiterpenes, one germacrane-type sesquiterpene, one carabrane-type sesquiterpene and one rareseco-sesquiterpenewere obtained and determined as granilin (1), telekin (2), isoalantolactone (3), 3α,5α-dihydroxyisoalantolactone (4), isotelekin (5), asperilin (6), 11(13)-dehydroivaxillin (7), carabrone (8), and Postia secoguaianolide (9), along with two monoterpenes 7,8-epoxy-9-(isobutyryloxy)thymolisobutyrate (10) and 10-hydroxy-8,9-dioxyisopropylidenethymol (11). Conclusion Compounds 9 and 10 are isolated from the genus Carpesium for the first time, compounds 3, 4, 6, and 10 are separated from C. abrotanoides for the first time. And it is the first time to report the 13C-NMR of compounds 4 and 6. Compound 1 shows cytotoxicity against HGC-27 cell and MDA-MB-231 cell with IC50 values of 18.18 μmol/L and 16.17 μmol/L. Compound 2 shows cytotoxicity against HGC-27 cell and MDA-MB-231 cell with IC50 values of 20.65 μmol/L and 17.62 μmol/L. Compound 8 shows cytotoxicity against HGC-27 cell with an IC50 value of 24.96 μmol/L.