Therapeutical effect of Curcuma aromatica ethylacetate extracts on myocardial ischemia reperfusion injury in rats / 中草药

ABSTRACT

Objective To investigate the effect of Curcuma aromatica ethylacetate extracts (CAEE) on myocardial ischemia reperfusion injury in rats. Methods The myocardial ischemia-reperfusion (I/R) injury model was established by the ligation and release of descending coronary artery in rats. I/R rats were equally divided into seven groups (i.e model group, four CAEE groups with 0.5, 1, 2, and 4 g/kg, two positive control groups with Di’ao Capsule or isosorbide mononitrate) with 10 rats in each group. In addition, 10 rats without I/R were arranged as Sham group. Each group was treated with corresponding drugs once daily for 7 d. The myocardial infarction area, cardiac function and cardiac geometric alteration were measured. Serum LDH and CK-MB were measured by chemical method and enzyme linked immunosorbent assays. The oxidative stress cytokines involving serum SOD, MDA, and GSH were measured by chemical methods. The myocardial tissue lesion was observed by histopathology. Results Compared with model group, myocardial infarction area was decreased in CAEE group with 1, 2, and 4 g/kg (crude drug), and the ejection fraction (EF) and the left ventricular pressure maximum rise/fall rates ( ± LVdp/dtmax) were increased in different degree treated with CAEE. Serum CK-MB and LDH were decreased in CAEE group. The oxidation/anti-oxidation balance can be adjusted by CAEE administration, the ratios of serum SOD/MDA and GSH/MDA were increased accompanying with trend increasing of SOD and GSH. The light microscopic myocardial tissue edema levels and inflammatory cell infiltration were significantly lightened in the CAEE group than the model group. Conclusion CAEE showed therapeutic benefits on rats in myocardial ischemia-reperfusion injury via the potential mechanism of balance status of oxidation and anti-oxidation.