Study on extraction of DNA from animal cornu / 中草药

ABSTRACT

Objective: To establish the method of DNA extraction for animal cornu, ensuring the quality of extracted DNA to meet the requirement of PCR and sequencing, which can be used in DNA extraction for the old animal horn samples. Methods: To avoid incomplete DNA extraction or damaged DNA by using EDTA decalcify when drawing material from bone, DTT, cell lysis solution and proteinase K were used to treat horn cells when drawing material from horny layer, other steps of DNA extraction were operated according to the reagent kit instructions. The effects of sampling amount and DTT dosage on the quality of DNA from horny layer were also investigated. Results: The sampling amount was 25 mg, DTT dosage was 20 μL, horn cells can be lysised completely, and the quality of DNA of all samples can meet the requirements of PCR. Conclusion: The method of DNA extraction from animal cornu established in this study can extract DNA completely, ensure standardized operation, and could be applied to the extraction of various animal horns.