Study on HPLC-DAD-ELSD fingerprint of Ilex kudingcha / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 5000-5004, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-852364
ABSTRACT
Objective In this paper, HPLC-DAD-ELSD technique was used to establish the chromatography fingerprint of Ilex kudingcha. Methods Shimadzu C18 column (250 mm × 4.6 mm, 5 μm) was used. Acetonitrile-water as the mobile phase; The flow speed was 1.0 mL/min. The detection wavelength was 210 nm and the column temperature was 35 ℃. Results The chromatography fingerprint of Ilex kudingcha from 10 different origins was established. In the chromatography fingerprint with HPLC-DAD of Ilex kudingcha, 18 common peaks were damarcated and the similarities of Ilex kudingcha were between 0.911-0.970, and the chromatography fingerprint with HPLC-ELSD of Ilex kudingcha, 11 common peaks were damarcated and the similarities of Ilex kudingcha were between 0.914-0.962. Chlorogenic acid, kaempferol-3-O-β-D-rhamnoside, isorhamnetin-3-O-β- D-rhamnoside, kudinoside C, kudinoside A, kudinoside E, kudinoside D were confired by LC-MS and binding control. Conclusion The method of precision, reproducibility, stability is accurate, which can be used as the evaluation of the quality of Ilex kudingcha.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Traditional and Herbal Drugs
Año:
2017
Tipo del documento:
Artículo
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