Analysis of chemical constituents in Liujing Toutong Tablets by HPLC-Q-TOF/MS / 中草药

ABSTRACT

Objective To separate and identify the chemical constituents in Liujing Toutong Tablets (LTT) by HPLC-Q-TOF/MS. Methods The main components were separated by using the gradient elution method with RP-HPLC, Diamonsil C18 (250 mm × 4.6 μm, 5 μm). The positive and negative electro spray ionization (ESI) source was adopted for determine the chromatographic effluent, the main chromatographic peaks are assigned and distinguished by Q-TOF. These components were further analyzed by accurately relative molecular mass of compounds, and Combined with Positive and negative ions MS information and related literature data. Results According to the MS principle, the corresponding reference substances and literature datas, Ninety-five compounds of LTT were identified, mainly including isoflavones, coumarins, iridoids and phthalides. Furthermore, all of the constituents were surveyed and classified according to their medicinal materials derivation. Conclusion In this study, the main components in LTT were elucidated completely by HPLC-Q-TOF/MS. The results could provide the evidence for the research on active constituents and quality control of LTT.