Study on preparation and quality evaluation of standard decoction of Gentiana Radix based on traditional decocting process / 中草药

ABSTRACT

Objective To evaluate the extraction ratio, content determination, transfer ratio, and feature maps or fingerprints by establishing 15 batches of standard decoction of Gentiana Radix from five different places. Methods The Gentiana Radix was dissolved in water based on the traditional decoction process, the content of gentiopicroside was determined by UPLC-DAD, the transfer ratio, the extraction ratio, the fingerprints and the common peaks were evaluated by UPLC/Q-TOF-MS/MS. Results The determination of gentiopicroside of 15 batches of standard decoction was in the range of 1.57-2.81 mg/mL, the transfer ratio of gentiopicroside was in the range of 50.71%-67.70%, the average transfer ratio was 59.20%, and the standard deviation was 7.31%. The extraction ratio was in the range of 15.72%-25.52%, the average extraction ratio was 19.20% and the standard deviation was about 2.85%. Ten peaks of fingerprint were confirmed in this research, such as lilac glucoside, 1-(4-hydroxy-3-methoxy)-phenyl- 1,2,3-glycerol or isomer, loganate, secologanoside, gentiopicroside, naptho(2,3-c)furan-1(3H)-one-9-(acetyloxy)-6-methoxy-8- [(2,3,4-tri-O-acetyl-6-O-(2,3,4-tri-oacetyl-β-D-xylopyranosyl)-β-D-glucopyranosyl)oxy] or isomer, C46H54O28 (unknown compound), C46H54O27 (unknown compound), macrophylloside B, and isomacrophylloside. Conclusion The preparation method in this paper conforms to the traditional decoction method, the analyzed samples had representiveness, the batches fit the fingerprint research requirements, the content of quality evaluation covers a wide range and the data obtained are representative. It can be used for the standard preparation and quality evaluation of traditional Chinese medicine and providing basic data for the quality and the industrialization of dispensing granules.