Studies on plasma fingerprint of Danggui Shaoyao Powder by UPLC-UV / 中草药

ABSTRACT

Objective To establish the plasma fingerprint of Danggui Shaoyao Powder (DSP) for the analysis of the transitional components in rat plasma after administration of DSP extracts Methods Eleven batches of rat plasma were prepared after oral administration and the plasma fingerprint was established by UPLC-UV. The transitional components in rat plasma after administration of DSP extracts was analyzed by UPLC-Q/TOF-MS. Results The plasma fingerprints of common peaks for 11 batches of DSP were established to ascertain the optimized blood collection time, and the method was used to process the plasma. Fifteen common peaks were detected in plasma fingerprint, and the similarity were both higher than 0.933. The methodology of plasma pharmacochemistry was adopted to analyze the common peaks, and 15 transitional components, including 10 prototype components and five metabolites were identified. Conclusion The established plasma fingerprint of DSP provide the basis for the further study of transitional components.