Chemical constituents from seeds of Aesculus chinensis / 中草药

ABSTRACT

Objective: To study the chemical constituents from the 70% ethanolic extracts of Aesculus chinensis seeds. Methods: The compounds were isolated by D101 macroporous adsorptive resins, silica gel, Sephadex LH-20, and preparative-HPLC and their structures were identified by chemical methods and spectroscopic analyses. Results: Ten compounds were isolated from the 70% ethanolic extracts of A. chinensis seeds. They were 2R,3R-3,7,3'-trihydroxy-5'-methoxyflavane-5-O-β-glucopyranoside (1), quercetin-4'-O-β-D-glucopyranoside (2), quercetin-3'-O-β-D-glucoside (3), quercetin-3-O-[β-D-xylopyranosyl (1-2)]-β-D- glucopyranoside (4), quercetin-3-O-[β-D-glucopyranosyl (1-4)]-α-L-rhamnopyranoside (5), kaempferol-3-O-[β-D-glucopyranosyl (1-4)]-α-L-rhamnopyranoside (6), kaempferol-3-O-[β-D-xylopyranosyl (1-2)]-β-D-glucopyranoside (7), kaemferol-3-O-[β-D- xylopyranosyl (1-2)] [β-D-glucopyranosyl (1-3)]-β-D-glucopyranoside (8), (-)-epicatechin (9), and quercetin (10). Conclusion: Compounds 1 and 2 are obtained from genus Aesculus Linn. for the first time, and compounds 3-9 are isolated from A. chinensis for the first time.