Regulation of seabuckthorn seed oil on metabolic pathway disturbances on rats exposed to chronic unpredictable mild stress / 中草药

ABSTRACT

Objective: To analyze and explore the changes of the metabolites in serum and urine of the chronic unpredictable depression rat model (CUMS) rats disturbed by seabuckthorn seed oil with using nuclear magnetic resonance spectroscopy (1H-NMR) and KEGG database. Methods: CUMS was prepared and the rats seabuckthorn seed oil was ig given to intervene at the same time for four weeks. Then the serum and the urine of rats were collected to test by 1H-NMR based metabolomics approach which purpose was to analyze the changes of the metabolites. Finally, the KEGG database was searched to analyze metabolic pathway. Results: A total of 17 metabolites were identified as potential biomarkers in serum and urine for the CUMS model, of which nine metabolites were found in serum including higher levels of lipids, β-OH-butyrate, lactate, and N-acetyl glycoprotein and lower levels of alanine, betaine, trimethylamine N-oxide, α-glucose, and β-glucose and eight metabolites were found in urine including level increments for succinate, aspirate and level reductions for acetate, 2-oxoglutaric acid, citrate, TMA, betaine, and phenylalanine, compared with the negative control group (NS). After intervened by seabuckthorn seed oil, the above different metabolites have appeared different degree of callback, closing to NS. Conclusion: These metabolic responses to CUMS treatment by seabuckthorn seed oil seemed to be some alterations in energy metabolism, lipid metabolism, amino acid metabolism, methylamine metabolism, and synthesis of neurotransmitter.