Identification of DNA barcoding of common medicinal plants in Lamiaceae / 中草药

ABSTRACT

Objective: To screen a proper DNA barcoding suitable for common medicinal plants in Lamiaceae. Methods: The rDNA ITS sequence from 33 kinds of common medicinal plants in Lamiaceae and the chloroplast matK gene sequence from 40 kinds of common medicinal plants in Lamiaceae were amplified by PCR and sequenced. The interspecific and intraspecific Kimura 2-parameter distance (K2P) and the mutation sites in each sequence were calculated, and barcoding gap of sequence was evaluated by MEGA6.0. The phylogenetic trees were constructed on the basis of Neighbor-Joining method. Results: The total length of ITS sequence was 620-698 bp, the average G + C content was 62.8%, the chloroplast matK gene sequence length was 859-932 bp, the average G + C content was 34%, and the ITS sequence and matK gene sequence have obvious DNA barcoding gap, but the matK DNA barcoding gap was less than that of ITS gene sequence. From clustering analysis, the matK gene can identify the different species in Lamiaceae better than ITS sequence. Conclusion: matK sequence can be used as a preferred sequence to identify the species in Lamiaceae.