Chemical comparison on different parts of Angelica sinensis Radix based on NMR metabolomics / 中草药

ABSTRACT

Objective: To compare the chemical constituents in different parts of Angelica Sinensis Radix (ASR). Methods: In this study, NMR-based metabolomics approach was used to compare the chemical constituents of different parts in ASR. And the metabolites of different parts in ASR were analyzed using PCA, OPLS-DA, and univariate analysis. Results: Totally 36 metabolites were identified in the NMR spectra. Multivariate analysis revealed that higher levels of some compounds, such as valine and isoleucine, were present at the head of ASR, while the tail showed higher contents of Z-butylidenephthalide, cis-Z,Z'-3a.7a,7a.3a'- dihydroxyligustilide, etc. The body showed higher contents of tryptophan, coniferyl ferulate, and the levels of aspartic acid, Z-ligustilide, etc were present at higher levels in both head and tail. For some metabolites, such as alanine and arginine, no significant difference was observed among the three parts of ASR. Conclusion: Both the primary and secondary metabolites of ASR can be detected at the same time by 1H-NMR based metabolic profiling approach used in this study, and the results revealed in this study laid the foundation for the further investigation of the relationship between the chemical components and biological effects of different parts in ASR.