Chemical constituents from Paris thibetica and their antitumor activities / 中草药

ABSTRACT

Objective: To investigate the chemical constituents from Paris thibetica and their antitumor activities. Methods: The separations and purifications were taken by column chromatography over silica gel, Sephadex LH-20, ODS-C18, and semi-preparative HPLC. The in vitro antitumor activities of the isolated compounds were studied by MTT method. Results: Fourteen compounds were isolated from ethanol extract of the rhizomes of P. thibetica, which were identified as paris saponin V (1), paris saponin I (2), paris saponin II (3), paris saponin VI (4), pennogennin-3-O-α-L-arabinofuranosyl-(1→4)-β-D-glucopyranoside (5), paris saponin H (6), pennogennin-3-O-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (7), paris saponin VII (8), parisyunnanoside G (9), trikamsteroside E (10), stigmasterol-3-O-β-D-glucopyranoside (11), β-sitosteryl palmitate (12), β-amyrin (13), and 4-hydroxy-5-methylfuran-3-carboxylic acid (14). Conclusion: All compounds are firstly reported from P. thibetica, and this is the first report of compounds 10 and 12-14 from the genus Paris L. Compounds 1-8 show cytotoxicities against BEL-7402. Among the tested compounds, compound 3 exhibits the strongest cytotoxicity with IC50 value of 0.48 μmol/L.