Study on fingerprints of Wuzi Yanzong Prescription-medicinal materials based on peak pattern matching / 中草药

ABSTRACT

Objective: To establish the HPLC-DAD fingerprints of Wuzi Yanzong Prescription (WYP) and to identify the characteristic peaks ion and preparations-medicinal materials peak pattern matching. Methods: The HPLC-DAD analysis was carried out using acetonitrile-0.4% phosphate acid aqueous solution at the flow out on a Waters Symmetry® C18 column (250 mm × 4.6 mm, 5 μm), using a mobile phase of rate of 1.0 mL/min. The detection wavelength was set at 254 nm and column temperature was maintained at 35 ℃. Similarity on 12 batches of WYP was estimated, and peak pattern matching of the original medicine was conducted. Results: The results showed that 24 common peaks were defined as follows The peaks No. 1 was gallic acid, No. 4 was geniposidic acid, No. 8 was chlorogenic acid, No.19 was hyperin, No. 20 was isoquercitrin, No. 21 was acteoside, No. 22 was kaempferol 3-rutinoside, and No. 23 was isoacteoside. The peak pattern matching showed that WYP had 8 (No. 2, 3, 6, 7, 8, 11, 12, and 18), 10 (No. 6, 7, 8, 9, 10, 15, 16, 19, 20, and 24), 7 (No. 1, 3, 7, 13, 14, 17, and 22), 3 (No. 4, 21, 23), and 1 (No. 3) matching peaks with Lycium barbarum, Cuscuta chinensis, Rubus chingii, Plantago asiatica, and Schisandra chinensis, respectively. The fingerprint similarity among the samples was all over 0.959. The fingerprint similarity between the chromatographic control fingerprint and samples was all over 0.979. Conclusion: Similarity evaluation combined with the peak matching of fingerprint for WYP could provide the scientific and simplicity methods for its identification and quality control.