UPLC characteristic chromatographic profile of Carthamus tinctorrius / 中草药

ABSTRACT

Objective: To establish fingerprint analysis method by UPLC for the quality control of Carthamus tinctorrius, and provide comprehensive evaluation of their quality in different regions and different varieties. Methods: The software "Similarity Evaluation System for Chromatographic Fingerprint of TCMs" (Version 2010A) was employed to generate the mean chromatogram and carry out the similarity analysis of the samples. Cluster analysis was adopted in combination with principal component analysis to study 30 batches from Xinjiang C. tinctorrius characteristic common peaks and to differentiate the Carthamus tinctorrius L resources. Results: A specific UPLC fingerprint of C. tinctorrius from Xinjiang province had been set up and establelished and 26 common peaks were designated. The results showed that the qualities of 30 batches of samples from Xinjiang province and four sets from Henan have significant differences and the samples collected different varieties from same region and differnent regions both had certainly differences. The difference among chromatographic fingerprints of C. tinctorrius samples were identified by cluster analysis and principal component analysis. Conclusion: UPLC fingerprint is an available, convenient, and reliable method, which can be used to access the quality of C. tinctorrius.