1H-NMR-based metabonomics study on toxicity alleviation effect of Bovis Calculus Artificialis, Gypsum Fibrosum, and Borneolum Synthcticum to Realgar (As2S2) in Niuhuang Jiedu Tablet / 中草药

ABSTRACT

Objective: To study the toxicity alleviation effect of Bovis Calculus Artificialis, Gypsum Fibrosum, and Borneolum Synthcticum to Realgar in Niuhuang Jiedu Tablet based on 1H-NMR metabonomics. Methods: Forty-two male Wistar rats were divided into six groups group A (control group), group B (treated with realgar), group C (treated with Niuhuang Jiedu Tablet), group D (treated with Niuhuang Jiedu Tablet without Bovis Calculus Artificialis), group E (treated with Niuhuang Jiedu Tablet without Gypsum Fibrosum), and group F (treated with Niuhuang Jiedu Tablet without Borneolum Synthcticum). Based on 1H-NMR spectra of urine and serum from rats, PLS-DA was performed to identify different metabolic profiles. Results: The metabolic profiles of group B were different from control group, while the metabolic profiles of all other groups were almost in line with those of control group. Conclusion: The toxicity alleviation effects of Bovis Calculus Artificialis, Gypsum Fibrosum, and Borneolum Synthcticum to Realgar in Niuhuang Jiedu Tablet are not obvious.