Evaluation and effects on HaCaT cells in vitro of tanshinone IIA-loaded nanostructured lipid carrier / 中草药

ABSTRACT

Objective: To evaluate TIIA-NLC's quality in vitro and study its effects on the HaCaT cells. Methods: Its particle size, polydispersity index (PDI) and photostability were investigated by Zetasizer analyzer and high-performance liquid chromatography (HPLC). The in vitro release of TIIA-NLC within 72 h was measured by dialysis method; Meanwhile MTT assay was used to detect proliferation activity on HaCaT cells. Results: The average particle size, PDI and Zeta potential of TIIA-NLC was (178 ± 9) nm, 0.183 ± 0.017 and (−27.5 ± 5.6) mV. The 72 h in vitro accumulative release rate was 52.28%, and the degradation velocity of TIIA was significantly slowed down. The TIIA-NLC inhibited the proliferation of HaCaT cells in concentration-dependent mode within a certain range. Compared with TIIA group, TIIA-NLC had more effects on the HaCaT cells. Conclusion: The prepared TIIA-NLC showed good stability, sustained release of interest, good cell biology intermiscibility, and could obviously increase the effects on the HaCaT cells.