Effect on the expression of key enzymes DS and P450 genes in the medicinal composition synthesis of Panax notoginseng under Cadmium stress / 中草药

ABSTRACT

Objective: To learn the effect on the expression of key enzymes DS and P450 genes in the medicinal composition synthesis of Panax notoginseng under exogenous Cadmium (Cd). Methods: Nine treatments of Cd (0.0, 0.1, 0.3, 0.6, 1.0, 3.0, 6.0, 10.0, and 30.0 mg∙kg-1) was added to the cultivated soil of Panax notoginseng, respectively. Two years later, mature Panax notoginseng was sampled. The expression of DS and P450 genes in root was detected using qRT-PCR method, and GAPDH gene was taken as the reference genes to calculate the relative expression of DS and P450 genes. The correlation between expression of key enzymes genes and main medicinal composition (notoginsenoside R1, ginsenoside Rb1, ginsenoside Rg1, and total saponins) was determined by correlation analysis. Results: Cd increased DS expression in low concentration, and inhibited in high levels, while with the adding Cd increasing, the P450 expression decreased significantly. However, the expression of DS and P450 genes didn't show good correlation with notoginsenoside R1, ginsenoside Rb1, ginsenoside Rg1, and total saponins (P > 0.5). Conclusion: Cd can affect the expression of DS and P450 genes in different ways and for the same gene, the expression is also various in different Cd levels. However, the main medicinal composition content wasn't influenced consequently.