Cloning and analysis of rDNA ITS sequences from seven kinds of medicinal plants in Goodyera L / 中草药

ABSTRACT

Objective: To identify the medicinal plants in Goodyera L. at molecular level, cloning and sequence analysis of seven internal transcribed spacer (ITS) sequences from seven plant species of Goodyera L. were performed. Methods: ITS sequences were cloned using PCR amplification, and sequence analysis, evaluation of genetic distance as well as construction of phylogenetic tree were conducted by bioinformatics software. Results: Full ITS sequences of seven kinds of plants in Goodyera L. were 700-702 bp in length. The length of ITS1 and ITS2 were 239-240 bp and 298-300 bp, respectively, while 5.8S sequences were more conserved with identical length of 162 bp. Thirty-four parsimony information sites among 107 variable sites were detected in the sequence. Genetic distances among seven plant species of Goodyera L. were 0.006-0.110. The greatest genetic distances were observed between V. grypoceras and V. diffusa var. brevibarbata indicating the farthest of their relative distinct relationship, and the smallest existed between V. diffusa and V. acuminata revealing their closest relationship. Conclusion: ITS sequences of seven kinds of medicinal plants in Goodyera L. are obtained with a result of rich information sites which could be used to distinguish these seven species completely.