Antipyretic mechanism of Xinhuang Tablet on fever rabbits induced by milk / 中草药

ABSTRACT

Objective: To investigate the mechanism of Xinhuang Tablet on fever rabbits induced by milk. Methods: Using the milk-reduced rabbit fever model to research the antipyretic mechanism of Xinhuang Tablet, blood plasma and hypothalamus were collected to measure the indicators related to body temperature, such as IL-1α, IL-1β, IL-6, TNF-α, PGE2, cAMP, cGMP, AVP, and Na+, K+-ATP ase. Results: In the model of milk-reduced rabbit fever, Xinhuang Tablet can significantly decline the contents of IL-1α, IL-1β, IL-6, PGE2, and TNF-α in the hypothalamus, decline the content of cAMP and ratio of cAMP/cGMP, decline the energy of Na-K-ATPase, and increase the variety of body temperature regulation related factors, such as content of AVP, in order to play a role of antipyretic. Conclusion: The antipyretic mechanism and adjustment of Xinhuang Tablet were associated with body temperature regulation related factors in the hypothalamus, such as IL-1α, IL-1β, IL-6, PGE2, TNF-α, cAMP, cAMP/cGMP, Na+, K+-ATP ase, and AVP; Its indomethacin can decline the contents of IL-1α, IL-6, PGE2, and TNF-α, increase the content of AVP, decline the energy of Na+, K+-ATP ase which plays the antipyretic effects of non-steroidal anti-inflammatory drugs, and the containing Chinese medicines can further decline the contents of IL-1β, IL-6, PGE2, and cAMP as well as the ratio of cAMP/cGMP in the hypothalamus of rabbits, synergy with indomethacin, in order to extend the indomethacin's antipyretic action time and enhance the indomethacin's strength.