Application of fingerprint combined with quantitative analysis of multi-component by single marker in quality evaluation of Ampelopsis grossedentata / 中草药

ABSTRACT

Objective: A new quality evaluation method using HPLC fingerprint coupled with quantitative analysis of multi-components by single marker method was developed for the quality control of Ampelopsis grossedentata. Methods: HPLC analysis was performed on an Agilent Eclipse Plus C18 (250 mm × 4.6 mm, 5 μm) column using methanol-0.05% aqueous solution of phosphoric acid as mobile phase at flow rate of 0.8 mL/min. The column temperature was set at 50 ℃ and the detection wavelength was at 258, 292, and 369 nm. The quality analyses of samples from different origins were accomplished by cluster analysis and principal component analysis using SPSS 19.0 statistical software. Results: The HPLC fingerprint of A. grossedentata from different regions was established. Twenty-four common peaks were found in the HPLC fingerprint of 14 samples, three important common components were identified as dihydromyricetin, myricitrin and myricetin. Further, the quantitative analysis of multi-components by single marker method of these contents was established. Conclusion: The method of HPLC fingerprint combined with quantitative analysis of multi-components by single marker method can be used for the quality control of A. grossedentata.