In silico cloning and bioinformatics analysis of cyclophilin from Lotus corniculatus / 中草药

ABSTRACT

Objective: Using electronic cloning technology to predict cyclophilin gene of Lotus corniculatus. Methods: Using glycine max cyclophilin sequence as probe sequence, based on EST sequence from NCBI and assembled by CAP3 sequence assembly programme, using bioinformatic database and related software, the structure prediction and function analysis were performed. Results: The full length of cyclophilin gene was 1 346 bp, it contained a 771 bp ORF, encoding 256 amino acids, and the protein was a hydrophilic protein. Conclusion: The study is intended to further explain the molecular genetic function theory and experimental basis.