Determination of asperosaponin VI, psoralen, and angelicin in Xianling Gubao Capsule by HPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2868-2870, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-853339
ABSTRACT
Objective:
To determine asperosaponin VI, psoralen, and angelicin in Xianling Gubao Capsules (XGC) via multi- wavelength HPLC method.Methods:
Separation was carried out on Welch Ultimate® XB-C18 column. The mobile phase was acetonitrile-water system and a linear gradient elution was used. The column temperature was 30℃. The detection wavelength for asperosaponin VI was set at 212 nm, those for psoralen and angelicin were set at 246 nm.Results:
Three components reached baseline separation, the linearity was good when sample volumes were in the ranges of 144.1-5 764.0 for asperosaponin VI (r = 0.999 6), 5.4-215.2 (r = 0.998 0) for psoralen, and 6.6-265.6 ng (r = 0.998 5) for angelicin. The average recoveries of asperosaponin VI, psoralen, and psoralen were 98.11%, 97.86%, and 98.22%, respectively. The RSDs of recoveries were all less than 2.0%.Conclusion:
The method is simple and accurate and has good separation, with high sensitivity and good efficiency for the determination of more-index components in XGC.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Traditional and Herbal Drugs
Año:
2016
Tipo del documento:
Artículo
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