Research on HPLC-ELSD fingerprint of Anemarrhenae Rhizoma / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2747-2750, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-853380
ABSTRACT
Objective:
To provide the HPLC-ELSD fingerprint analysis method of Anemarrhenae Rhizoma, and to reflect and comprehensively control the quality of Anemarrhenae Rhizoma.Methods:
The HPLC-ELSD separation was performed on a C8 analytical column (4.6 mm × 250 mm, 5 μm) gradient eluted with a mixture consisting of acetonitrile (A) and 0.2% acetic acid-water (B) at a flow rate of 1 mL/min with ELSD detector. Using a linear gradient of 0 min, 5% A; 5 min, 7% A; 5-15 min, 15% A; 15-36 min, 22% A; 36-43 min, 35% A; 43 min, 40% A; 51 min, 50% A; 51-60 min, 100% A. The temperature of column was 25 ℃. The flow rate of ELSD detector atomizer (air) was 2.6 mL/min. The temperature of the drift tube was 100 ℃ and the injection volume was 20 μL.Results:
The fingerprint with better separation effect by using gradient elution method within 60 min was established, and also the 12 peak which are common peak were determined. The most of chemical components of Anemarrhenae Rhizoma were chromatographic separated in this HPLC-ELSD fingerprint.Conclusion:
The method is simple and reliable, and in this way for the steroidal saponins and flavonoids of Anemarrhenae Rhizoma can be identified at the same time. Moreover, it can fully reflect the characteristics of the chemical composition of Anemarrhenae Rhizoma, therefore it can be used as an effective quality control method for Anemarrhenae Rhizoma.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Traditional and Herbal Drugs
Año:
2016
Tipo del documento:
Artículo
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