Transport research on water extract from Magnoliae Officinalis Cortex across Caco-2 monolayer model / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2313-2318, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-853433
ABSTRACT
Objective:
To evaluate the transport property of water extract from Magnoliae Officinalis Cortex (WEMOC) across Caco-2 monolayer model by bioactivity method and observe the influence on the activity of P-glycoprotein (P-gp) in cell membrane.Methods:
The validated Caco-2 cell monolayer model was used to observe the bidirection transportation of WEMOC; The amount of WEMOC in the receiver compartment, apparent permeability coefficient (P'app), and efflux ratio (ER) value could be determined according to the standard curve of total anti-oxidant capacity. The accumulation and efflux of rhodamine123 (Rh123), which is the substrate of P-gp, were determined to evaluate the influence of WEMOC on P-gp.Results:
P'app(AP→BL) of WEMOC was around 1 × 10-5 cm/s, and positively related to concentration; ER was far less than 2; It had no impact on the accumulation and efflux of Rh123.Conclusion:
The active ingredient groups of WEMOC with anti-oxidant activity can be well absorbed by passive diffusion down a concentration gradient. Neither is it the substrate of P-gp, nor does it have influence on P-gp activity. Our research establishes a novel method to evaluate the transport characteristics of WEMOC, which provides the pharmacokinetic basis for the clinical application of Magnoliae Officinalis Cortex.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Traditional and Herbal Drugs
Año:
2016
Tipo del documento:
Artículo
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