Analysis on chemical constituents from Glycyrrhizae Radix et Rhizoma by HPLC-Q-TOF-MS / 中草药

ABSTRACT

Objective: To analyze the main chemical constituents of Glycyrrhizae Radix et Rhizoma by HPLC-Q-TOF-MS in the positive and negative ions mode. Methods: The chromatographic fingerprint was obtained with Diamonsil II C18 column (250 mm×4.6 mm, 5 μm) and gradient elution with 0.05% H2O-formic acid (A)-acetonitrile (B), and the flow rate was 1 mL/min. The column temperature was maintained at 35℃. The detection wavelength was of 200-600 nm. Positive and negative ions MS information of Bruker Daltonics 1200 HPLC-Q-TOF was coupled with elemental analysis and compared with the literature data to analyze the compounds information. Results: Combined with the accurate relative molecular mass of compounds provided by HPLC-Q-TOF-MS, 40 compounds were identificated in the ethanolic extract from Glycyrrhizae Radix et Rhizoma, which include 28 flavonoids, 11 triterpenoids saponins, and one coumarin. Conclusion: The method provides the technical support for the quality control of Glycyrrhizae Radix et Rhizoma, and contributes the reference data to elucidating the potential basis of Glycyrrhizae Radix et Rhizoma